Hotspot

trnN-GUU-ndhF

IRb · 109205-110287 · 1083 bp

Back to Calystegia

Pi 0.00185

MarkerSeek score 59.90

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1083
variable_sites: 3
pi: 0.00185
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	AGGATTCGAACCTACGACCA	TGGGATATGCGAGAATTCCT	50.0	45.0	58.2	56.1	1228 bp	1.0	63.6
trnN-GUU-ndhF_p2	CCCCAAGTAGGATTCGAACC	TGGGATATGCGAGAATTCCT	55.0	45.0	57.7	56.1	1236 bp	1.0	62.2
trnN-GUU-ndhF_p3	AGGATTCGAACCTACGACCA	TGGGATATGCGAGAATTCCTT	50.0	42.9	58.2	56.9	1228 bp	1.0	61.8
trnN-GUU-ndhF_p4	AGGATTCGAACCTACGACCA	TTGGGATATGCGAGAATTCCT	50.0	42.9	58.2	56.9	1229 bp	1.0	61.8
trnN-GUU-ndhF_p5	CCCCAAGTAGGATTCGAACC	TGGGATATGCGAGAATTCCTT	55.0	42.9	57.7	56.9	1236 bp	1.0	60.4