Hotspot

trnG-GCC-trnfM-CAU

LSC · 37920-38186 · 267 bp

Back to Calystegia

Pi 0.00749

MarkerSeek score 44.30

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 267
variable_sites: 3
pi: 0.00749
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	AGAAGATGCGGGTTCGATTC	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.1	58.0	346-368 bp	1.0	84.0
trnG-GCC-trnfM-CAU_p2	GAGAAGATGCGGGTTCGATT	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.1	58.0	347-369 bp	1.0	84.0
trnG-GCC-trnfM-CAU_p3	AGAAGATGCGGGTTCGATTC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	310-332 bp	1.0	84.0
trnG-GCC-trnfM-CAU_p4	GAGAAGATGCGGGTTCGATT	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	311-333 bp	1.0	84.0
trnG-GCC-trnfM-CAU_p5	ATGCGGATATGGTCGAATGG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	57.9	58.0	387-409 bp	1.0	83.6