Hotspot

rpl16

Genome · 82175-83437 · 1263 bp

Back to Calyptothecium

Pi 0.00317
MarkerSeek score 59.80
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
Genome
length_bp
1263
variable_sites
4
pi
0.00317
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 CGTACAACTACTGCTCGGAC GGAGACCGCAAACTTCGTAT 55.0 50.0 58.1 58.0 2034 bp 1.0 79.3
rpl16_p2 ACAGGACCAAAAACACGTGT GGAGACCGCAAACTTCGTAT 45.0 50.0 58.2 58.0 2148 bp 1.0 78.8
rpl16_p3 TCGTACAACTACTGCTCGGA GGAGACCGCAAACTTCGTAT 50.0 50.0 58.2 58.0 2035 bp 1.0 78.6
rpl16_p4 CCACTGTTGTCGGCTACATT GGAGACCGCAAACTTCGTAT 50.0 50.0 58.2 58.0 1887 bp 1.0 78.6
rpl16_p5 GGACCAAAAACACGTGTTCC GGAGACCGCAAACTTCGTAT 50.0 50.0 57.8 58.0 2145 bp 1.0 78.6