Hotspot

rbcL

Genome · 59599-61026 · 1428 bp

Back to Calyptothecium

Pi 0.0042

MarkerSeek score 49.20

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: Genome
length_bp: 1428
variable_sites: 6
pi: 0.0042
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TCATAGCAACAGCTCGAACC	CTTCATCCATAGGCTGCCAG	50.0	55.0	58.0	58.1	2887-2888 bp	1.0	79.4
rbcL_p2	CATAGCAACAGCTCGAACCT	CTTCATCCATAGGCTGCCAG	50.0	55.0	58.0	58.1	2886-2887 bp	1.0	79.4
rbcL_p3	CACTCATAGCAACAGCTCGA	CTTCATCCATAGGCTGCCAG	50.0	55.0	57.7	58.1	2890-2891 bp	1.0	77.2
rbcL_p4	CCCACTCATAGCAACAGCTC	CTTCATCCATAGGCTGCCAG	55.0	55.0	58.3	58.1	2892-2893 bp	1.0	76.8
rbcL_p5	TTGAGTTGTAGGGAGGGACC	CTTCATCCATAGGCTGCCAG	55.0	55.0	58.3	58.1	2182-2183 bp	1.0	76.8