Hotspot

chlL

Genome · 114062-114937 · 876 bp

Back to Calyptothecium

Pi 0.00228

MarkerSeek score 60.10

Primer available yes

Label chlL

Feature Metadata

label_name: chlL
feature_type: gene
region: Genome
length_bp: 876
variable_sites: 2
pi: 0.00228
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlL_p1	CCTGTTTCGCATTCAAAAGTGA	AGATTCTTTAATATGACCATAGAACA	40.9	26.9	58.0	54.3	1041 bp	1.0	40.2
chlL_p2	GTGTGGTAATTACCTGTTTCGC	AGATTCTTTAATATGACCATAGAACA	45.5	26.9	57.9	54.3	1053 bp	1.0	40.2
chlL_p3	ACCTGTTTCGCATTCAAAAGT	AGATTCTTTAATATGACCATAGAACA	38.1	26.9	56.9	54.3	1042 bp	1.0	40.2
chlL_p4	TGTGGTAATTACCTGTTTCGCA	AGATTCTTTAATATGACCATAGAACA	40.9	26.9	58.3	54.3	1052 bp	1.0	40.2
chlL_p5	ACCTGTTTCGCATTCAAAAGTG	AGATTCTTTAATATGACCATAGAACA	40.9	26.9	58.3	54.3	1042 bp	1.0	40.2