Hotspot

cemA

Genome · 63852-65369 · 1518 bp

Back to Calyptothecium

Pi 0.00198

MarkerSeek score 59.80

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: Genome
length_bp: 1518
variable_sites: 3
pi: 0.00198
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	TTTCCGTTTTAGCGAATCTT	ACTCTACATTAGTAATCAAAAGAACT	35.0	26.9	53.5	54.5	1622 bp	1.0	40.1
cemA_p2	TTTTCCGTTTTAGCGAATCT	ACTCTACATTAGTAATCAAAAGAACT	35.0	26.9	53.5	54.5	1623 bp	1.0	40.1
cemA_p3	TTTTCCGTTTTAGCGAATCTT	ACTCTACATTAGTAATCAAAAGAACT	33.3	26.9	54.4	54.5	1623 bp	1.0	40.1
cemA_p4	TTTTTCCGTTTTAGCGAATCT	ACTCTACATTAGTAATCAAAAGAACT	33.3	26.9	54.4	54.5	1624 bp	1.0	40.1
cemA_p5	TTTTTCCGTTTTAGCGAATCTT	ACTCTACATTAGTAATCAAAAGAACT	31.8	26.9	55.2	54.5	1624 bp	1.0	40.1