Hotspot

petD-rps11

Genome · 79500-79819 · 320 bp

Back to Calyptothecium

Pi 0.0219

MarkerSeek score 65.80

Primer available yes

Label petD-rps11

Feature Metadata

label_name: petD-rps11
feature_type: igs
region: Genome
length_bp: 320
variable_sites: 7
pi: 0.0219
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rps11_p1	GCCGTATCCCTTTGGTTAGG	AGCTATTCGTCGGAGTGGTA	55.0	50.0	58.0	58.0	488-489 bp	1.0	80.6
petD-rps11_p2	CTTTTCGTCGTCCAGTAGCA	AGCTATTCGTCGGAGTGGTA	50.0	50.0	57.9	58.0	532-533 bp	1.0	80.3
petD-rps11_p3	CGTCCAGTAGCAACAACAGT	AGCTATTCGTCGGAGTGGTA	50.0	50.0	58.1	58.0	524-525 bp	1.0	79.8
petD-rps11_p4	GTCGTCCAGTAGCAACAACA	AGCTATTCGTCGGAGTGGTA	50.0	50.0	58.1	58.0	526-527 bp	1.0	79.7
petD-rps11_p5	TCGTCCAGTAGCAACAACAG	AGCTATTCGTCGGAGTGGTA	50.0	50.0	57.9	58.0	525-526 bp	1.0	79.6