Hotspot

psaI-ycf4

Genome · 62828-63179 · 352 bp

Back to Calyptothecium

Pi 0.0341
MarkerSeek score 60.50
Primer available yes
Label psaI-ycf4

Feature Metadata

label_name
psaI-ycf4
feature_type
igs
region
Genome
length_bp
352
variable_sites
12
pi
0.0341
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaI-ycf4_p1 TTGAACAAGACGAGATAATCT GCTCTACCCAAAGCCATTCA 33.3 50.0 52.3 57.9 409-421 bp 1.0 42.3
psaI-ycf4_p2 ATTGAACAAGACGAGATAATCT GCTCTACCCAAAGCCATTCA 31.8 50.0 53.0 57.9 410-422 bp 1.0 42.3
psaI-ycf4_p3 TGAACAAGACGAGATAATCTAA GCTCTACCCAAAGCCATTCA 31.8 50.0 52.4 57.9 408-420 bp 1.0 42.3
psaI-ycf4_p4 TTGAACAAGACGAGATAATCTA GCTCTACCCAAAGCCATTCA 31.8 50.0 52.4 57.9 409-421 bp 1.0 42.3
psaI-ycf4_p5 TTGAACAAGACGAGATAATCT AAAAACCCGAATGCACCAAA 33.3 40.0 52.3 57.0 482-494 bp 1.0 42.0