Hotspot

atpE-atpB

Genome · 57620-57625 · 6 bp

Back to Calyptothecium

Pi 0.167

MarkerSeek score 87.50

Primer available yes

Label atpE-atpB

Feature Metadata

label_name: atpE-atpB
feature_type: igs
region: Genome
length_bp: 6
variable_sites: 1
pi: 0.167
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 87.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpE-atpB_p1	CGGCCACTGTTTGTAGATGA	AGGCTGCAACCTTACAAGTG	50.0	50.0	57.9	58.4	124 bp	1.0	76.8
atpE-atpB_p2	TCGGCCACTGTTTGTAGATG	AGGCTGCAACCTTACAAGTG	50.0	50.0	57.9	58.4	125 bp	1.0	76.8
atpE-atpB_p3	CGGCCACTGTTTGTAGATGA	GCTGCAACCTTACAAGTGGA	50.0	50.0	57.9	58.4	122 bp	1.0	76.7
atpE-atpB_p4	TCGGCCACTGTTTGTAGATG	GCTGCAACCTTACAAGTGGA	50.0	50.0	57.9	58.4	123 bp	1.0	76.7
atpE-atpB_p5	CGGCCACTGTTTGTAGATGA	TGAAGCTACTGCAAAGGCTG	50.0	50.0	57.9	58.5	138 bp	1.0	76.0