Hotspot

ndhI-rps15

SSC · 115880-117754 · 1875 bp

Back to Calypso

Pi 0.0201
MarkerSeek score 61.00
Primer available yes
Label ndhI-rps15

Feature Metadata

label_name
ndhI-rps15
feature_type
igs
region
SSC
length_bp
1875
variable_sites
37
pi
0.0201
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhI-rps15_p1 TGGACATATGCGAACACATACT TCTTGGGAAAACGTCAACGA 40.9 45.0 57.6 57.7 2018-2104 bp 1.0 60.9
ndhI-rps15_p2 ATTGGACATATGCGAACACA TCTTGGGAAAACGTCAACGA 40.0 45.0 55.4 57.7 2020-2106 bp 1.0 59.6
ndhI-rps15_p3 TTGGACATATGCGAACACAT TCTTGGGAAAACGTCAACGA 40.0 45.0 55.4 57.7 2019-2105 bp 1.0 59.6
ndhI-rps15_p4 AGGTCTATTGGACATATGCGA TCTTGGGAAAACGTCAACGA 42.9 45.0 56.3 57.7 2026-2112 bp 1.0 58.7
ndhI-rps15_p5 TGGACATATGCGAACACATAC TCTTGGGAAAACGTCAACGA 42.9 45.0 56.1 57.7 2018-2104 bp 1.0 57.3