Hotspot

rpl32-trnL-UAG

SSC · 110602-111258 · 657 bp

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Pi 0.0188

MarkerSeek score 68.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 657
variable_sites: 12
pi: 0.0188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TCCACCGGACAGTCAAAAAG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	738-756 bp	1.0	81.9
rpl32-trnL-UAG_p2	TCCACCGGACAGTCAAAAAG	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.0	57.8	731-749 bp	1.0	81.5
rpl32-trnL-UAG_p3	TCCACCGGACAGTCAAAAAG	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	58.0	57.8	737-755 bp	1.0	81.3
rpl32-trnL-UAG_p4	TCCACCGGACAGTCAAAAAG	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.0	57.8	736-754 bp	1.0	81.3
rpl32-trnL-UAG_p5	TCCACCGGACAGTCAAAAAG	GAGCAGCGTGTCTACCAATT	50.0	50.0	58.0	58.3	732-750 bp	1.0	80.7