Hotspot

trnN-GUU-rpl32

SSC · 109045-110427 · 1383 bp

Back to Calypso

Pi 0.0426

MarkerSeek score 85.60

Primer available yes

Label trnN-GUU-rpl32

Feature Metadata

label_name: trnN-GUU-rpl32
feature_type: igs
region: SSC
length_bp: 1383
variable_sites: 52
pi: 0.0426
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 85.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rpl32_p1	AGTCAGTTAACAGCCAACCG	GCTTTTACTGCCTCTAAAGATCC	50.0	43.5	58.1	57.3	1457-1518 bp	1.0	55.9
trnN-GUU-rpl32_p2	ACGACCAGTCAGTTAACAGC	GCTTTTACTGCCTCTAAAGATCC	50.0	43.5	57.8	57.3	1463-1524 bp	1.0	55.6
trnN-GUU-rpl32_p3	AGGATTCGAACCTACGACCA	GCTTTTACTGCCTCTAAAGATCC	50.0	43.5	58.2	57.3	1476-1537 bp	1.0	55.6
trnN-GUU-rpl32_p4	AGTCAGTTAACAGCCAACCG	AGAAAAAGCTTTTACTGCCTCT	50.0	36.4	58.1	56.2	1464-1525 bp	1.0	55.3
trnN-GUU-rpl32_p5	GGATTCGAACCTACGACCAG	GCTTTTACTGCCTCTAAAGATCC	55.0	43.5	57.8	57.3	1475-1536 bp	1.0	55.2