Hotspot

rpl36-infA

LSC · 79430-79521 · 92 bp

Back to Calypso

Pi 0.0326

MarkerSeek score 71.20

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: LSC
length_bp: 92
variable_sites: 3
pi: 0.0326
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	GACTATAATTCGCCCTCGCC	TGATTCAACCAGGGGACGTA	55.0	50.0	58.3	58.4	225 bp	1.0	75.9
rpl36-infA_p2	GACTATAATTCGCCCTCGCC	CCGGCGCAGTTTTATACGTA	55.0	50.0	58.3	58.4	285 bp	1.0	75.0
rpl36-infA_p3	GCCTACGAATCAAGCGACAT	TGATTCAACCAGGGGACGTA	50.0	50.0	58.4	58.4	208 bp	1.0	74.9
rpl36-infA_p4	TGACTATAATTCGCCCTCGC	TGATTCAACCAGGGGACGTA	50.0	50.0	57.6	58.4	226 bp	1.0	74.8
rpl36-infA_p5	GCCTACGAATCAAGCGACAT	CCGGCGCAGTTTTATACGTA	50.0	50.0	58.4	58.4	268 bp	1.0	73.9