Hotspot

psbB-psbT

LSC · 73287-73703 · 417 bp

Back to Calypso

Pi 0.0635

MarkerSeek score 89.60

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 417
variable_sites: 25
pi: 0.0635
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 89.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	TCAGAGATGTTTTTGCCGGT	TGGAACTTTAGGTGGTTCTCG	45.0	47.6	57.7	57.6	611-618 bp	1.0	72.9
psbB-psbT_p2	TCAGAGATGTTTTTGCCGGT	GGAACTTTAGGTGGTTCTCGA	45.0	47.6	57.7	57.3	610-617 bp	1.0	70.7
psbB-psbT_p3	GCCAGAACCTTGTTCAGAGA	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	57.4	57.6	624-631 bp	1.0	70.5
psbB-psbT_p4	GCTCAAGTGGAATTTGGAGC	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	57.4	57.6	573-580 bp	1.0	70.3
psbB-psbT_p5	GCCAGAACCTTGTTCAGAGA	GGAACTTTAGGTGGTTCTCGA	50.0	47.6	57.4	57.3	623-630 bp	1.0	68.3