Hotspot

psbL-psbF

LSC · 63532-63553 · 22 bp

Back to Calypso

Pi 0.0455

MarkerSeek score 74.10

Primer available yes

Label psbL-psbF

Feature Metadata

label_name: psbL-psbF
feature_type: igs
region: LSC
length_bp: 22
variable_sites: 1
pi: 0.0455
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbL-psbF_p1	CATTCGGGTTTGATTGTGTCA	GGACTGGCTGTACCTACTGT	42.9	55.0	57.1	58.4	110 bp	1.0	61.9
psbL-psbF_p2	TCATTCGGGTTTGATTGTGTC	GGACTGGCTGTACCTACTGT	42.9	55.0	56.8	58.4	111 bp	1.0	59.7
psbL-psbF_p3	TGTTCATTCGGGTTTGATTGTG	GGACTGGCTGTACCTACTGT	40.9	55.0	57.8	58.4	114 bp	1.0	59.3
psbL-psbF_p4	GTTCATTCGGGTTTGATTGTGT	GGACTGGCTGTACCTACTGT	40.9	55.0	57.8	58.4	113 bp	1.0	59.3
psbL-psbF_p5	CATTCGGGTTTGATTGTGTCAT	GGACTGGCTGTACCTACTGT	40.9	55.0	57.6	58.4	110 bp	1.0	57.7