Hotspot

accD-psaI

LSC · 58052-58796 · 745 bp

Back to Calypso

Pi 0.0179

MarkerSeek score 65.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 745
variable_sites: 13
pi: 0.0179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CACGGTTTCCTTCCCTTGAA	AAAGAAGCCATTGCGATTGC	50.0	45.0	58.0	58.0	834-852 bp	1.0	82.7
accD-psaI_p2	TTTGACCCAATCGTACCACG	AAAGAAGCCATTGCGATTGC	50.0	45.0	58.2	58.0	894-912 bp	1.0	81.1
accD-psaI_p3	CACGGTTTCCTTCCCTTGAA	CGATTGCCGGAAAGACTAGG	50.0	55.0	58.0	58.4	821-839 bp	1.0	79.4
accD-psaI_p4	TGACCCAATCGTACCACGTA	AAAGAAGCCATTGCGATTGC	50.0	45.0	58.5	58.0	892-910 bp	1.0	79.1
accD-psaI_p5	CACGGTTTCCTTCCCTTGAA	CGGAAAGACTAGGCCCACTA	50.0	55.0	58.0	58.5	814-832 bp	1.0	78.7