Hotspot

atpH-atpI

LSC · 14387-15154 · 768 bp

Back to Calypso

Pi 0.013

MarkerSeek score 66.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 768
variable_sites: 10
pi: 0.013
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CGCAATACCTTCTACGGCTT	AACTTTGGCTGCGGCTTATA	50.0	45.0	58.1	57.9	937-943 bp	1.0	79.4
atpH-atpI_p2	TCCGATAGAAGCAAGCCCTA	AACTTTGGCTGCGGCTTATA	50.0	45.0	57.9	57.9	886-892 bp	1.0	79.4
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	AACTTTGGCTGCGGCTTATA	45.0	45.0	57.9	57.9	865-871 bp	1.0	78.8
atpH-atpI_p4	CCGATAGAAGCAAGCCCTAC	AACTTTGGCTGCGGCTTATA	55.0	45.0	57.8	57.9	885-891 bp	1.0	78.8
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	AACTTTGGCTGCGGCTTATA	50.0	45.0	57.8	57.9	864-870 bp	1.0	78.3