Hotspot

trnA-UGC-trnI-GAU

Genome · 117135-117212 · 78 bp

Back to Calypogeia

Pi 0.0385
MarkerSeek score 70.90
Primer available yes
Label trnA-UGC-trnI-GAU

Feature Metadata

label_name
trnA-UGC-trnI-GAU
feature_type
igs
region
Genome
length_bp
78
variable_sites
3
pi
0.0385
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnA-UGC-trnI-GAU_p1 TAGGAGCAACGACCCAATTG TTCAAGTCCAGGATAGCCCA 50.0 50.0 57.9 58.0 153 bp 1.0 80.4
trnA-UGC-trnI-GAU_p2 GTAGGAGCAACGACCCAATT TTCAAGTCCAGGATAGCCCA 50.0 50.0 57.9 58.0 154 bp 1.0 80.4
trnA-UGC-trnI-GAU_p3 TAGGAGCAACGACCCAATTG AGGTCTCTGGTTCAAGTCCA 50.0 50.0 57.9 57.9 163 bp 1.0 79.8
trnA-UGC-trnI-GAU_p4 TAGGAGCAACGACCCAATTG TGTATGCTGTGTTCGGGAAG 50.0 50.0 57.9 57.9 241 bp 1.0 79.4
trnA-UGC-trnI-GAU_p5 GTAGGAGCAACGACCCAATT TGTATGCTGTGTTCGGGAAG 50.0 50.0 57.9 57.9 242 bp 1.0 79.4