Hotspot

rps15-ycf1

Genome · 104440-106135 · 1696 bp

Back to Calypogeia

Pi 0.0266

MarkerSeek score 61.00

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: Genome
length_bp: 1696
variable_sites: 45
pi: 0.0266
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	ATGGGTGAATTCTTTGGCAT	TGGAGGTTATGAGAGAATTGA	40.0	38.1	55.5	53.7	1756-1760 bp	1.0	42.2
rps15-ycf1_p2	ATGGGTGAATTCTTTGGCAT	TCAAAATGGAGGTTATGAGAGA	40.0	36.4	55.5	54.5	1762-1766 bp	1.0	42.2
rps15-ycf1_p3	ATGGGTGAATTCTTTGGCAT	TCAAAATGGAGGTTATGAGAGAA	40.0	34.8	55.5	55.3	1762-1766 bp	1.0	42.2
rps15-ycf1_p4	ATGGGTGAATTCTTTGGCAT	ATGGAGGTTATGAGAGAATTGA	40.0	36.4	55.5	54.3	1757-1761 bp	1.0	42.2
rps15-ycf1_p5	ATGGGTGAATTCTTTGGCAT	TGGAGGTTATGAGAGAATTGAT	40.0	36.4	55.5	54.3	1756-1760 bp	1.0	42.2