Hotspot

ndhH-rps15

Genome · 104144-104172 · 29 bp

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Pi 0.103

MarkerSeek score 77.60

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: Genome
length_bp: 29
variable_sites: 3
pi: 0.103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	GGTGAGGGCCCATACTTACT	GGTCAGCGTAAACGTCTTCT	55.0	50.0	58.5	57.9	186 bp	1.0	76.9
ndhH-rps15_p2	GTGGTGAGGGCCCATACTTA	GGTCAGCGTAAACGTCTTCT	55.0	50.0	58.8	57.9	188 bp	1.0	74.5
ndhH-rps15_p3	TGGTGAGGGCCCATACTTAC	GGTCAGCGTAAACGTCTTCT	55.0	50.0	58.8	57.9	187 bp	1.0	74.5
ndhH-rps15_p4	GGTGAGGGCCCATACTTACT	ATTGGGTCAGCGTAAACGTC	55.0	50.0	58.5	58.6	190 bp	1.0	72.8
ndhH-rps15_p5	GGTGAGGGCCCATACTTACT	TATTGGGTCAGCGTAAACGT	55.0	45.0	58.5	57.3	191 bp	1.0	71.3