Hotspot

ndhl-ndhA

Genome · 101044-101148 · 105 bp

Back to Calypogeia

Pi 0.0505

MarkerSeek score 70.70

Primer available yes

Label ndhl-ndhA

Feature Metadata

label_name: ndhl-ndhA
feature_type: igs
region: Genome
length_bp: 105
variable_sites: 5
pi: 0.0505
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhl-ndhA_p1	TGTGGAATCCTCGCTGATTG	GCAGACAGAATCTCGTTGGT	50.0	50.0	58.0	57.9	1254-1260 bp	1.0	80.7
ndhl-ndhA_p2	GTGTGGAATCCTCGCTGATT	GCAGACAGAATCTCGTTGGT	50.0	50.0	58.0	57.9	1255-1261 bp	1.0	80.7
ndhl-ndhA_p3	TGTGGAATCCTCGCTGATTG	GAGGCTGGCATTTTCCCTTA	50.0	50.0	58.0	57.9	839-845 bp	1.0	80.7
ndhl-ndhA_p4	TGTGGAATCCTCGCTGATTG	TTAGGAGGCTGGCATTTTCC	50.0	50.0	58.0	57.9	843-849 bp	1.0	80.7
ndhl-ndhA_p5	GTGTGGAATCCTCGCTGATT	GAGGCTGGCATTTTCCCTTA	50.0	50.0	58.0	57.9	840-846 bp	1.0	80.7