Hotspot

trnI-GAU-trnA-UGC

Genome · 86224-86301 · 78 bp

Back to Calypogeia

Pi 0.0385
MarkerSeek score 70.90
Primer available yes
Label trnI-GAU-trnA-UGC

Feature Metadata

label_name
trnI-GAU-trnA-UGC
feature_type
igs
region
Genome
length_bp
78
variable_sites
3
pi
0.0385
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-GAU-trnA-UGC_p1 TTCAAGTCCAGGATAGCCCA TAGGAGCAACGACCCAATTG 50.0 50.0 58.0 57.9 153 bp 1.0 80.4
trnI-GAU-trnA-UGC_p2 TTCAAGTCCAGGATAGCCCA GTAGGAGCAACGACCCAATT 50.0 50.0 58.0 57.9 154 bp 1.0 80.4
trnI-GAU-trnA-UGC_p3 AGGTCTCTGGTTCAAGTCCA TAGGAGCAACGACCCAATTG 50.0 50.0 57.9 57.9 163 bp 1.0 79.8
trnI-GAU-trnA-UGC_p4 TGTATGCTGTGTTCGGGAAG TAGGAGCAACGACCCAATTG 50.0 50.0 57.9 57.9 241 bp 1.0 79.4
trnI-GAU-trnA-UGC_p5 TGTATGCTGTGTTCGGGAAG GTAGGAGCAACGACCCAATT 50.0 50.0 57.9 57.9 242 bp 1.0 79.4