Hotspot

petA-psbJ

Genome · 64176-64309 · 134 bp

Back to Calypogeia

Pi 0.0703

MarkerSeek score 73.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 134
variable_sites: 9
pi: 0.0703
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TTTTTCCGATTGTTTCGCCG	CCGAATAAACAGAGCGTGGA	45.0	50.0	58.0	58.0	954 bp	0.5	59.6
petA-psbJ_p2	TTTTTCCGATTGTTTCGCCG	TCCGAATAAACAGAGCGTGG	45.0	50.0	58.0	58.0	955 bp	0.5	59.6
petA-psbJ_p3	ACAAGATCCGTTGCGTATCC	CCGAATAAACAGAGCGTGGA	50.0	50.0	58.1	58.0	599 bp	0.5	59.5
petA-psbJ_p4	ACAAGATCCGTTGCGTATCC	TCCGAATAAACAGAGCGTGG	50.0	50.0	58.1	58.0	600 bp	0.5	59.5
petA-psbJ_p5	CAAGATCCGTTGCGTATCCA	CCGAATAAACAGAGCGTGGA	50.0	50.0	58.1	58.0	598 bp	0.5	59.4