Hotspot

trnR-CCG-accD

Genome · 59323-59589 · 267 bp

Back to Calypogeia

Pi 0.0262
MarkerSeek score 57.20
Primer available yes
Label trnR-CCG-accD

Feature Metadata

label_name
trnR-CCG-accD
feature_type
igs
region
Genome
length_bp
267
variable_sites
7
pi
0.0262
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-CCG-accD_p1 GAGGTCAAGGGTTCGAATCC AAGCTCCGATTAATCCACCA 55.0 45.0 58.0 56.6 357-358 bp 1.0 70.2
trnR-CCG-accD_p2 GAGGTCAAGGGTTCGAATCC AGCTCCGATTAATCCACCAA 55.0 45.0 58.0 56.6 356-357 bp 1.0 70.2
trnR-CCG-accD_p3 AAGCATGAGGTCAAGGGTTC AAGCTCCGATTAATCCACCA 50.0 45.0 57.8 56.6 363-364 bp 1.0 68.7
trnR-CCG-accD_p4 AAGCATGAGGTCAAGGGTTC AGCTCCGATTAATCCACCAA 50.0 45.0 57.8 56.6 362-363 bp 1.0 68.7
trnR-CCG-accD_p5 GAAGCATGAGGTCAAGGGTT AGCTCCGATTAATCCACCAA 50.0 45.0 57.8 56.6 363-364 bp 1.0 68.7