Hotspot

trnV-UAC-trnM-CAU

Genome · 54922-55092 · 171 bp

Back to Calypogeia

Pi 0.0819
MarkerSeek score 80.20
Primer available yes
Label trnV-UAC-trnM-CAU

Feature Metadata

label_name
trnV-UAC-trnM-CAU
feature_type
igs
region
Genome
length_bp
171
variable_sites
14
pi
0.0819
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-UAC-trnM-CAU_p1 TCTACCGCTGAGCTATAGCC TTGAACCAATGACTCTCGCC 55.0 50.0 58.5 58.2 250 bp 1.0 79.2
trnV-UAC-trnM-CAU_p2 TTTCGATAACCATTGGCGCA TTGAACCAATGACTCTCGCC 45.0 50.0 58.5 58.2 286 bp 1.0 78.0
trnV-UAC-trnM-CAU_p3 TTTTCGATAACCATTGGCGC TTGAACCAATGACTCTCGCC 45.0 50.0 57.4 58.2 287 bp 1.0 77.9
trnV-UAC-trnM-CAU_p4 TCTACCGCTGAGCTATAGCC CAATGACTCTCGCCGTATGA 55.0 50.0 58.5 57.5 244 bp 1.0 76.9
trnV-UAC-trnM-CAU_p5 GTGTAAACGAGGCGCTCTAC TTGAACCAATGACTCTCGCC 55.0 50.0 58.7 58.2 265 bp 1.0 76.8