Hotspot

trnT-UGU-trnL-UAA

Genome · 51551-51820 · 270 bp

Back to Calypogeia

Pi 0.0407

MarkerSeek score 64.20

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: Genome
length_bp: 270
variable_sites: 11
pi: 0.0407
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GACCTCTGAGCTAAACAGGC	ACCAATTTCGCCATACTCCC	55.0	50.0	58.0	57.9	310 bp	1.0	82.3
trnT-UGU-trnL-UAA_p2	TACAAGTGCGATGCTCTGAC	ACCAATTTCGCCATACTCCC	50.0	50.0	58.0	57.9	327 bp	1.0	82.2
trnT-UGU-trnL-UAA_p3	GACCTCTGAGCTAAACAGGC	TAGCGTCTACCAATTTCGCC	55.0	50.0	58.0	58.1	318 bp	1.0	82.1
trnT-UGU-trnL-UAA_p4	TACAAGTGCGATGCTCTGAC	TAGCGTCTACCAATTTCGCC	50.0	50.0	58.0	58.1	335 bp	1.0	82.0
trnT-UGU-trnL-UAA_p5	GCATTACAAGTGCGATGCTC	ACCAATTTCGCCATACTCCC	50.0	50.0	57.9	57.9	331 bp	1.0	81.5