Hotspot

trnH-GUG-ycf2

Genome · 30312-31165 · 854 bp

Back to Calypogeia

Pi 0.0328

MarkerSeek score 67.70

Primer available yes

Label trnH-GUG-ycf2

Feature Metadata

label_name: trnH-GUG-ycf2
feature_type: igs
region: Genome
length_bp: 854
variable_sites: 27
pi: 0.0328
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-ycf2_p1	TTGGGTATTAGCACCATGGC	AACTTTCTGGAGATTCGCCC	50.0	50.0	57.9	57.9	1908-1927 bp	1.0	82.5
trnH-GUG-ycf2_p2	CCTTATGCATCCGTTCCACA	AACTTTCTGGAGATTCGCCC	50.0	50.0	58.0	57.9	2194-2213 bp	1.0	82.4
trnH-GUG-ycf2_p3	AAGGCAGTGGATTGTGGATC	AACTTTCTGGAGATTCGCCC	50.0	50.0	57.9	57.9	1558-1577 bp	1.0	82.4
trnH-GUG-ycf2_p4	ATCTGGTTCACTGCTTTGGG	AACTTTCTGGAGATTCGCCC	50.0	50.0	58.1	57.9	1923-1942 bp	1.0	82.3
trnH-GUG-ycf2_p5	GCCAAGTGGATTAAGGCAGT	AACTTTCTGGAGATTCGCCC	50.0	50.0	58.2	57.9	1570-1589 bp	1.0	82.2