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petN-trnC-GCA

Genome · 5601-5953 · 353 bp

Back to Calypogeia

Pi 0.0482

MarkerSeek score 68.60

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: Genome
length_bp: 353
variable_sites: 17
pi: 0.0482
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCACCCCAAGCTATATTAGT	CTGCAAATCCTTCATCCCCA	42.9	50.0	56.2	57.9	422-424 bp	1.0	59.5
petN-trnC-GCA_p2	AGCACCCCAAGCTATATTAGT	TGCAAATCCTTCATCCCCAG	42.9	50.0	56.2	57.9	421-423 bp	1.0	59.5
petN-trnC-GCA_p3	AGCACCCCAAGCTATATTAGT	ACTGCAAATCCTTCATCCCC	42.9	50.0	56.2	57.9	423-425 bp	1.0	59.5
petN-trnC-GCA_p4	AGCACCCCAAGCTATATTAGT	GCAAATCCTTCATCCCCAGT	42.9	50.0	56.2	57.9	420-422 bp	1.0	59.5
petN-trnC-GCA_p5	AGCACCCCAAGCTATATTAGT	GTAAGGCAAGGGACTGCAAA	42.9	50.0	56.2	58.4	435-437 bp	1.0	57.5