Hotspot

rps7-ndhB

Genome · 1635-1769 · 135 bp

Back to Calypogeia

Pi 0.0519

MarkerSeek score 70.10

Primer available yes

Label rps7-ndhB

Feature Metadata

label_name: rps7-ndhB
feature_type: igs
region: Genome
length_bp: 135
variable_sites: 7
pi: 0.0519
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7-ndhB_p1	GCTTTTGCGCATTTTCGTTA	TGGAATACATTCGGGTAAAATTGT	40.0	33.3	56.5	56.9	219-220 bp	1.0	42.6
rps7-ndhB_p2	GCTTTTGCGCATTTTCGTTA	CAAAAATTGGAATACATTCGGGT	40.0	34.8	56.5	56.0	226-227 bp	1.0	42.5
rps7-ndhB_p3	GCTTTTGCGCATTTTCGTTA	AAATTGGAATACATTCGGGT	40.0	35.0	56.5	52.8	223-224 bp	1.0	42.5
rps7-ndhB_p4	GCTTTTGCGCATTTTCGTTAA	CAAAAATTGGAATACATTCGGGT	38.1	34.8	57.2	56.0	226-227 bp	1.0	42.5
rps7-ndhB_p5	GCTTTTGCGCATTTTCGTTA	AAAATTGGAATACATTCGGGT	40.0	33.3	56.5	53.7	224-225 bp	1.0	42.5