Hotspot

ndhG

SSC · 119476-120006 · 531 bp

Back to Calycanthus

Pi 0.01

MarkerSeek score 51.20

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 8
pi: 0.01
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	AACAATAGCCGGCCCAATAG	TTTGTGGGAACTGTGTCGAG	50.0	50.0	58.0	58.1	1632 bp	1.0	79.9
ndhG_p2	AACAATAGCCGGCCCAATAG	CAGAGCGTTTTCGTGGTAGA	50.0	50.0	58.0	57.9	1800 bp	1.0	79.8
ndhG_p3	AACAATAGCCGGCCCAATAG	TCAGAGCGTTTTCGTGGTAG	50.0	50.0	58.0	57.9	1801 bp	1.0	79.8
ndhG_p4	ACCATGTTTCGGCTTGTGAT	TTTGTGGGAACTGTGTCGAG	45.0	50.0	58.1	58.1	1472 bp	1.0	79.4
ndhG_p5	ACCATGTTTCGGCTTGTGAT	CAGAGCGTTTTCGTGGTAGA	45.0	50.0	58.1	57.9	1640 bp	1.0	79.3