Hotspot

trnN(guu)-trnR(acg)

IRa · 131000-131602 · 603 bp

Back to Calycanthus

Pi 0.00553

MarkerSeek score 62.30

Primer available yes

Label trnN(guu)-trnR(acg)

Feature Metadata

label_name: trnN(guu)-trnR(acg)
feature_type: igs
region: IRa
length_bp: 603
variable_sites: 5
pi: 0.00553
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN(guu)-trnR(acg)_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	744-748 bp	1.0	78.8
trnN(guu)-trnR(acg)_p2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	55.0	55.0	58.0	57.7	781-785 bp	1.0	78.2
trnN(guu)-trnR(acg)_p3	CGGTTGGCTGTTAACTGACT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	714-718 bp	1.0	77.9
trnN(guu)-trnR(acg)_p4	GCTGTTAACTGACTGGTCGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	708-712 bp	1.0	77.6
trnN(guu)-trnR(acg)_p5	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	695-699 bp	1.0	77.6