Hotspot

clpP-psbB

LSC · 73606-74040 · 435 bp

Back to Calycanthus

Pi 0.0046

MarkerSeek score 58.20

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 435
variable_sites: 3
pi: 0.0046
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	GGCCCAACCAGAAACTAGAG	50.0	55.0	58.0	57.9	613 bp	1.0	79.5
clpP-psbB_p2	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	531 bp	1.0	78.5
clpP-psbB_p3	CTTTTGGAACACCAATGGGC	GGCCCAACCAGAAACTAGAG	50.0	55.0	57.8	57.9	559 bp	1.0	78.2
clpP-psbB_p4	CGCACTATACGTCAACCCAA	GTATAGAGCCATCGAACCGG	50.0	55.0	58.0	57.7	631 bp	1.0	77.6
clpP-psbB_p5	TTTGGAACACCAATGGGCAT	GGCCCAACCAGAAACTAGAG	45.0	55.0	58.3	57.9	557 bp	1.0	77.3