Hotspot

petA-psbJ

LSC · 64413-65604 · 1192 bp

Back to Calycanthus

Pi 0.0123

MarkerSeek score 61.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1192
variable_sites: 22
pi: 0.0123
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTTTGTTGT	ATTCCGGATTGGGATCATCC	45.0	50.0	58.2	57.1	1330-1333 bp	1.0	72.9
petA-psbJ_p2	ACGTGTCCAAGGTTTGTTGT	ACTGGTATTCCTGTGATCGG	45.0	50.0	58.2	56.7	1379-1382 bp	1.0	69.7
petA-psbJ_p3	ACGTGTCCAAGGTTTGTTGT	CTGGTATTCCTGTGATCGGT	45.0	50.0	58.2	56.7	1378-1381 bp	1.0	69.7
petA-psbJ_p4	ACGTGTCCAAGGTTTGTTGT	GGTTCATATTCCGGATTGGGA	45.0	47.6	58.2	57.5	1337-1340 bp	1.0	67.9
petA-psbJ_p5	ACGTGTCCAAGGTTTGTTGT	ACTGGTATTCCTGTGATCGGT	45.0	47.6	58.2	58.5	1379-1382 bp	1.0	67.6