Hotspot

trnT(ggu)-psbD

LSC · 32613-34094 · 1482 bp

Back to Calycanthus

Pi 0.0156
MarkerSeek score 73.00
Primer available yes
Label trnT(ggu)-psbD

Feature Metadata

label_name
trnT(ggu)-psbD
feature_type
igs
region
LSC
length_bp
1482
variable_sites
33
pi
0.0156
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.95
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT(ggu)-psbD_p1 GTGGTAGAGTAACGCCATGG GAAACGGTCCCTCCTTAACC 55.0 55.0 58.1 57.9 1581-1624 bp 1.0 83.9
trnT(ggu)-psbD_p2 TCAGTGGTAGAGTAACGCCA GAAACGGTCCCTCCTTAACC 50.0 55.0 58.1 57.9 1584-1627 bp 1.0 83.7
trnT(ggu)-psbD_p3 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1589-1632 bp 1.0 82.8
trnT(ggu)-psbD_p4 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1592-1635 bp 1.0 82.6
trnT(ggu)-psbD_p5 GTGGTAGAGTAACGCCATGG CGAAACGGTCCCTCCTTAAC 55.0 55.0 58.1 58.3 1582-1625 bp 1.0 82.5