Hotspot

trnK(uuu)-rps16

LSC · 4208-5010 · 803 bp

Back to Calycanthus

Pi 0.00416

MarkerSeek score 61.60

Primer available yes

Label trnK(uuu)-rps16

Feature Metadata

label_name: trnK(uuu)-rps16
feature_type: igs
region: LSC
length_bp: 803
variable_sites: 5
pi: 0.00416
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK(uuu)-rps16_p1	AAAGCCGAGTACTCTACCGT	AAAAGGGCGCTCAACCTAC	50.0	52.6	58.2	58.1	932-934 bp	1.0	70.8
trnK(uuu)-rps16_p2	AAGCCGAGTACTCTACCGTT	AAAAGGGCGCTCAACCTAC	50.0	52.6	58.2	58.1	931-933 bp	1.0	70.8
trnK(uuu)-rps16_p3	CCGCACTTAAAAGCCGAGTA	AAAAGGGCGCTCAACCTAC	50.0	52.6	58.3	58.1	941-943 bp	1.0	69.9
trnK(uuu)-rps16_p4	AAAGCCGAGTACTCTACCGT	GAAAAGGGCGCTCAACCTAC	50.0	55.0	58.2	59.2	933-935 bp	1.0	69.7
trnK(uuu)-rps16_p5	AAGCCGAGTACTCTACCGTT	GAAAAGGGCGCTCAACCTAC	50.0	55.0	58.2	59.2	932-934 bp	1.0	69.7