Hotspot

ccsA

SSC · 125554-126513 · 960 bp

Back to Calotropis

Pi 0

MarkerSeek score 32.20

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 960
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	AGTAGCGGCATGTCATCTTC	ATGGTTCGTGCCCATTCAC	50.0	52.6	57.8	58.4	1139-1144 bp	1.0	66.7
ccsA_p2	GAGTAGCGGCATGTCATCTT	ATGGTTCGTGCCCATTCAC	50.0	52.6	57.8	58.4	1140-1145 bp	1.0	66.7
ccsA_p3	GTAGCGGCATGTCATCTTCT	ATGGTTCGTGCCCATTCAC	50.0	52.6	57.8	58.4	1138-1143 bp	1.0	66.7
ccsA_p4	AGTAGCGGCATGTCATCTTC	CATGGTTCGTGCCCATTCA	50.0	52.6	57.8	58.4	1140-1145 bp	1.0	66.7
ccsA_p5	GTAGCGGCATGTCATCTTCT	CATGGTTCGTGCCCATTCA	50.0	52.6	57.8	58.4	1139-1144 bp	1.0	66.7