Hotspot

accD

LSC · 64488-68819 · 4332 bp

Back to Calotropis

Pi 0.00639

MarkerSeek score 61.70

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 4332
variable_sites: 26
pi: 0.00639
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	ATGCGCAAGATAACGATGGT	CATACATAGTACGCCGGGTG	45.0	55.0	58.0	57.9	2452-2668 bp	1.0	83.9
accD_p2	TCGAATGATATCGATGCACAAGA	CGAGCTTGGATCTTTTGTCG	39.1	50.0	58.1	57.2	104-866 bp	1.0	54.3
accD_p3	CGAATGATATCGATGCACAAGA	CGAGCTTGGATCTTTTGTCG	40.9	50.0	56.8	57.2	103-865 bp	1.0	53.4
accD_p4	TCGAATGATATCGATGCACA	CGAGCTTGGATCTTTTGTCG	40.0	50.0	54.8	57.2	776-866 bp	1.0	52.8
accD_p5	TCGAATGATATCGATGCACAAG	CGAGCTTGGATCTTTTGTCG	40.9	50.0	56.8	57.2	776-866 bp	1.0	52.7