Hotspot

trnN-GUU-ycf1

IRb · 118393-119268 · 876 bp

Back to Calotropis

Pi 0

MarkerSeek score 60.70

Primer available yes

Label trnN-GUU-ycf1

Feature Metadata

label_name: trnN-GUU-ycf1
feature_type: igs
region: IRb
length_bp: 876
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ycf1_p1	AGGATTCGAACCTACCACCA	ATCCACAAAAGAGTCCGAGC	50.0	50.0	58.1	57.9	884-1018 bp	1.0	82.8
trnN-GUU-ycf1_p2	AGGATTCGAACCTACCACCA	CTAGGGAGAGTGTGCTCAGA	50.0	55.0	58.1	57.9	905-1039 bp	1.0	82.5
trnN-GUU-ycf1_p3	AGGATTCGAACCTACCACCA	TCCGAGCAAAACGACTGAAT	50.0	45.0	58.1	57.8	871-1005 bp	1.0	82.3
trnN-GUU-ycf1_p4	AGGATTCGAACCTACCACCA	AAAGAGTCCGAGCAAAACGA	50.0	45.0	58.1	57.8	877-1011 bp	1.0	81.7
trnN-GUU-ycf1_p5	AGGATTCGAACCTACCACCA	AGGGAGAGTGTGCTCAGAAA	50.0	50.0	58.1	58.3	903-1037 bp	1.0	81.4