Hotspot

rps7-rps12

IRb · 107406-108026 · 621 bp

Back to Calotropis

Pi 0
MarkerSeek score 59.50
Primer available yes
Label rps7-rps12

Feature Metadata

label_name
rps7-rps12
feature_type
igs
region
IRb
length_bp
621
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps7-rps12_p1 TTTTTCCTTCTGCAGCACCT CCCTAGATGCTGTCGGAGTA 45.0 55.0 57.9 58.0 703-708 bp 1.0 79.9
rps7-rps12_p2 TTTTTCCTTCTGCAGCACCT AGGATTTACCCGGTGTGAGA 45.0 50.0 57.9 58.1 742-747 bp 1.0 79.5
rps7-rps12_p3 GTTTTTCCTTCTGCAGCACC CCCTAGATGCTGTCGGAGTA 50.0 55.0 57.9 58.0 704-709 bp 1.0 79.3
rps7-rps12_p4 TTTTTCCTTCTGCAGCACCT GAGGGGGAAGGGTTAAGGAT 45.0 55.0 57.9 58.1 757-762 bp 1.0 79.2
rps7-rps12_p5 GTTTTTCCTTCTGCAGCACC AGGATTTACCCGGTGTGAGA 50.0 50.0 57.9 58.1 743-748 bp 1.0 79.0