Hotspot

matK-trnK-UUU

LSC · 3696-4383 · 688 bp

Back to Calotropis

Pi 0.00145

MarkerSeek score 59.60

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 688
variable_sites: 1
pi: 0.00145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ACTATGGTCATGGGCAAGTG	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.6	58.2	820 bp	1.0	75.3
matK-trnK-UUU_p2	ACTATGGTCATGGGCAAGTG	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.6	58.2	819 bp	1.0	75.3
matK-trnK-UUU_p3	GGTCATGGGCAAGTGCATAA	AACGGTAGAGTACTCGGCTT	50.0	50.0	58.5	58.2	815 bp	1.0	74.5
matK-trnK-UUU_p4	GGTCATGGGCAAGTGCATAA	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.5	58.2	814 bp	1.0	74.5
matK-trnK-UUU_p5	ACTATGGTCATGGGCAAGTG	GGGTTGCTAACTCAACGGTA	50.0	50.0	57.6	57.5	833 bp	1.0	73.0