Hotspot

rps16

LSC · 5246-6431 · 1186 bp

Back to Calophyllum

Pi 0.0121

MarkerSeek score 53.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1186
variable_sites: 21
pi: 0.0121
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	CCTTTCAGGATCAGTCGTGG	TCAAGCGGAGTTTTGCTCAT	55.0	45.0	58.0	58.1	2523-2554 bp	1.0	80.7
rps16_p2	CCTTTCAGGATCAGTCGTGG	ACCGGTTCCAAATTCAACGA	55.0	45.0	58.0	58.0	1943 bp	0.333333	53.0
rps16_p3	TCAGGATCAGTCGTGGTCTT	ACCGGTTCCAAATTCAACGA	50.0	45.0	58.1	58.0	1939 bp	0.333333	52.5
rps16_p4	TTCAGGATCAGTCGTGGTCT	ACCGGTTCCAAATTCAACGA	50.0	45.0	58.1	58.0	1940 bp	0.333333	52.5
rps16_p5	GCGTAAAAGATGTTAGCCGC	ACCGGTTCCAAATTCAACGA	50.0	45.0	57.9	58.0	1874 bp	0.333333	52.4