Hotspot

ndhF

SSC · 116452-118695 · 2244 bp

Back to Calophyllum

Pi 0.00297

MarkerSeek score 44.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2244
variable_sites: 10
pi: 0.00297
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CACCGGGGAAGAATCAAACT	TGGGATTTGTTTTTGACCGAC	50.0	42.9	57.8	57.3	2626-2784 bp	1.0	69.2
ndhF_p2	CACCGGGGAAGAATCAAACT	GGGATTTGTTTTTGACCGACT	50.0	42.9	57.8	57.0	2625-2783 bp	1.0	67.0
ndhF_p3	CACCGGGGAAGAATCAAACT	TTGGGATTTGTTTTTGACCGAC	50.0	40.9	57.8	58.0	2627-2785 bp	1.0	66.6
ndhF_p4	CACCGGGGAAGAATCAAACT	TGGGATTTGTTTTTGACCGA	50.0	40.0	57.8	55.8	2626-2784 bp	1.0	65.1
ndhF_p5	CCGGAAACTCGGTATCACAT	TGGGATTTGTTTTTGACCGAC	50.0	42.9	57.4	57.3	2536-2549 bp	0.666667	48.6