Hotspot

ndhF-rpl32

SSC · 118696-119667 · 972 bp

Back to Calophyllum

Pi 0.0113
MarkerSeek score 69.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
972
variable_sites
16
pi
0.0113
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGTTGCTTCCGGAAAAAGAAG TTCAAGGCTGCCCAATATCC 42.9 50.0 57.3 57.9 1143-1174 bp 1.0 68.7
ndhF-rpl32_p2 TGTTGCTTCCGGAAAAAGAAG CAAGGCTGCCCAATATCCTT 42.9 50.0 57.3 57.9 1141-1172 bp 1.0 68.7
ndhF-rpl32_p3 TGTTGCTTCCGGAAAAAGAAG AAAAAGCTTTCAAGGCTGCC 42.9 45.0 57.3 57.8 1151-1182 bp 1.0 67.3
ndhF-rpl32_p4 TGTTGCTTCCGGAAAAAGAA TTCAAGGCTGCCCAATATCC 40.0 50.0 56.1 57.9 1143-1174 bp 1.0 67.1
ndhF-rpl32_p5 TTGTTGCTTCCGGAAAAAGA TTCAAGGCTGCCCAATATCC 40.0 50.0 56.1 57.9 1144-1175 bp 1.0 67.1