Hotspot

trnR-CCG-trnN-GUU

IRb · 113444-114076 · 633 bp

Back to Calophyllum

Pi 0.0079

MarkerSeek score 63.70

Primer available yes

Label trnR-CCG-trnN-GUU

Feature Metadata

label_name: trnR-CCG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 633
variable_sites: 7
pi: 0.0079
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-CCG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	TCCTCCCATTGTTCCTCAGT	55.0	50.0	57.8	58.0	779 bp	1.0	78.6
trnR-CCG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	GCTGTTAACTGACTGGTCGT	55.0	50.0	57.8	57.8	738 bp	1.0	77.8
trnR-CCG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	725 bp	1.0	77.8
trnR-CCG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	GGGTTGAGAACGGGAATTGA	55.0	50.0	57.8	57.8	815 bp	1.0	77.4
trnR-CCG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	CTGGTCGTAGGTTCGAATCC	55.0	55.0	57.8	57.8	726 bp	1.0	77.4