Hotspot

accD-psaI

LSC · 62066-62675 · 610 bp

Back to Calophyllum

Pi 0.00773

MarkerSeek score 63.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 610
variable_sites: 7
pi: 0.00773
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CACGCTTTCTTTCCTTTGCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	730-743 bp	1.0	80.1
accD-psaI_p2	CCACGCTTTCTTTCCTTTGC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	731-744 bp	1.0	80.1
accD-psaI_p3	CACGCTTTCTTTCCTTTGCC	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.2	57.9	736-749 bp	1.0	79.5
accD-psaI_p4	CCACGCTTTCTTTCCTTTGC	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.2	57.9	737-750 bp	1.0	79.5
accD-psaI_p5	ACGCTTTCTTTCCTTTGCCT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.3	58.0	729-742 bp	1.0	79.5