Hotspot

ndhC-trnV-UAC

LSC · 53269-54526 · 1258 bp

Back to Calophyllum

Pi 0.0056

MarkerSeek score 63.60

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1258
variable_sites: 10
pi: 0.0056
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTGGTCCTTTGCTAATCGGG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.3	57.8	1399-1426 bp	1.0	77.2
ndhC-trnV-UAC_p2	GTCCTTTGCTAATCGGGGAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.4	57.8	1396-1423 bp	1.0	74.8
ndhC-trnV-UAC_p3	CTGGTCCTTTGCTAATCGGG	GAAGGTCTACGGTTCGAGTC	55.0	55.0	58.3	57.5	1398-1425 bp	1.0	74.5
ndhC-trnV-UAC_p4	TCTGGTCCTTTGCTAATCGG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.3	57.8	1400-1427 bp	1.0	74.4
ndhC-trnV-UAC_p5	CTGGTCCTTTGCTAATCGGG	GTTCGAGTCCGTATAGCCCT	55.0	55.0	58.3	58.7	1387-1414 bp	1.0	73.4