Hotspot

trnL-UAA-trnF-GAA

LSC · 50637-51049 · 413 bp

Back to Calophyllum

Pi 0.00807

MarkerSeek score 64.70

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 413
variable_sites: 5
pi: 0.00807
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	TCCGCCGACTTTAGAAATCG	TCTACCAGCTGAGCTATCCC	50.0	55.0	58.1	58.0	458-505 bp	1.0	84.1
trnL-UAA-trnF-GAA_p2	TCCGCCGACTTTAGAAATCG	TTTGAACTGGTGACACGAGG	50.0	50.0	58.1	58.1	494-541 bp	1.0	83.4
trnL-UAA-trnF-GAA_p3	TCCGCCGACTTTAGAAATCG	TCAGTCCTCTGCTCTACCAG	50.0	55.0	58.1	57.9	470-517 bp	1.0	82.9
trnL-UAA-trnF-GAA_p4	TAAGAGGAAAATCCGCCGAC	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.7	58.0	469-516 bp	1.0	82.3
trnL-UAA-trnF-GAA_p5	GTAAGAGGAAAATCCGCCGA	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.7	58.0	470-517 bp	1.0	82.2