Hotspot

psbC-trnS-UGA

LSC · 38406-38634 · 229 bp

Back to Calophyllum

Pi 0.0233

MarkerSeek score 67.30

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 229
variable_sites: 8
pi: 0.0233
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	TCATTTATGGCATGCGGGAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	57.9	58.1	406 bp	1.0	80.6
psbC-trnS-UGA_p2	TCATTTATGGCATGCGGGAA	GATGGCCGAGTGGTTGATAG	45.0	55.0	57.9	58.1	417 bp	1.0	80.0
psbC-trnS-UGA_p3	TCATTTATGGCATGCGGGAA	GGGTTCGAATCCCTCTCTCT	45.0	55.0	57.9	58.2	355 bp	1.0	79.6
psbC-trnS-UGA_p4	GCAGCAGGATTCGAAAAAGG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	57.7	58.1	372 bp	1.0	79.1
psbC-trnS-UGA_p5	GCAGCAGGATTCGAAAAAGG	GATGGCCGAGTGGTTGATAG	50.0	55.0	57.7	58.1	383 bp	1.0	78.5