Hotspot

rpoB-trnC-GCA

LSC · 28758-30011 · 1254 bp

Back to Calophyllum

Pi 0.00986

MarkerSeek score 63.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1254
variable_sites: 18
pi: 0.00986
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AGCTCTTCTCTTAAGCCCTGA	GCGTCTTTTGTTGATCAGGC	47.6	50.0	58.2	58.0	1442-1450 bp	1.0	71.6
rpoB-trnC-GCA_p2	AAGCCCTGATCAATGAACCT	GCGTCTTTTGTTGATCAGGC	45.0	50.0	56.8	58.0	1430-1438 bp	1.0	71.3
rpoB-trnC-GCA_p3	AGCTCTTCTCTTAAGCCCTGA	CCGGATTTGAACTGGGGAAA	47.6	50.0	58.2	58.1	1417-1425 bp	1.0	71.2
rpoB-trnC-GCA_p4	AAGCCCTGATCAATGAACCT	CCGGATTTGAACTGGGGAAA	45.0	50.0	56.8	58.1	1405-1413 bp	1.0	70.9
rpoB-trnC-GCA_p5	GCCCTGATCAATGAACCTACA	GCGTCTTTTGTTGATCAGGC	47.6	50.0	57.7	58.0	1428-1436 bp	1.0	70.7