Hotspot

atpH-atpI

LSC · 15248-16404 · 1157 bp

Back to Calophyllum

Pi 0.00723

MarkerSeek score 64.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1157
variable_sites: 12
pi: 0.00723
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACGGAAGCAGCAGAAATCA	CGAATCCATGGAGGGACATC	45.0	55.0	58.1	57.8	1205-1221 bp	1.0	79.1
atpH-atpI_p2	ACGGAAGCAGCAGAAATCAA	CGAATCCATGGAGGGACATC	45.0	55.0	58.1	57.8	1204-1220 bp	1.0	79.1
atpH-atpI_p3	GTCCAATAGAAGCAAGCCCA	CGAATCCATGGAGGGACATC	50.0	55.0	57.9	57.8	1245-1261 bp	1.0	78.9
atpH-atpI_p4	CCTTCTACAGCTTGACCTGC	CGAATCCATGGAGGGACATC	55.0	55.0	58.3	57.8	1288-1304 bp	1.0	77.7
atpH-atpI_p5	ACGGAAGCAGCAGAAATCAA	GCTGCTGCTTATATAGGCGA	45.0	50.0	58.1	57.6	1221-1237 bp	1.0	77.6